Homework 4 (revised 11/20)

<body topmargin=0 leftmargin=0 marginheight=0 marginwidth=0> <table cellpadding=0 cellspacing=0 border=0><tr> <td valign="top" colspan=2 height=110 BGCOLOR="#666699" TEXT="#080000" bgproperties="fixed" background="04a00840erlcjm.gif"> <div> <FONT SIZE="5" COLOR="#FFFFFF" FACE="Arial" ><B>Biology 2120 "Genetics"</B></FONT><B>     |     </B><A HREF="index.htm" TARGET="_top" TITLE="Biology 2120 Genetics"><U><B>home</B></U></A></div> <div> <A HREF="id27.htm" TARGET="_top" TITLE="Homework 1"><U>Homework 1</U></A>   |   <A HREF="id28.htm" TARGET="_top" TITLE="Homework 2 (MWF)"><U>Homework 2 (MWF)</U></A>   |   <A HREF="id45.htm" TARGET="_top" TITLE="Homework 2 (TR)"><U>Homework 2 (TR)</U></A>   |   <A HREF="id29.htm" TARGET="_top" TITLE=" Homework 3"><U> Homework 3</U></A>   |   <B>Homework 4 (revised 11/20)</B></div> <div> </div> </td> </tr><tr> <td valign="top" width=190 BGCOLOR="#CCCCFF" TEXT="#080000" background="04411c00egdysm.gif"> <bR> </td> <td valign="top" height=370 BGCOLOR="#FFFFFF" TEXT="#080000"> <div> Homework 4 (revised 11/20)</div> <div> December 3 for TR and December 4 for MWF</div> <div> 1. Gene Regulation in Bacteria</div> <div> <IMG SRC="131292c0.gif" border=0 width="297" height="44" ALIGN="BOTTOM" HSPACE="0" VSPACE="0"></div> <div> <IMG SRC="130cb580.gif" border=0 width="203" height="88" ALIGN="BOTTOM" HSPACE="0" VSPACE="0"></div> <div> An operon in the bacterium that causes the black plague is the <I>omp</I> operon.  It is regulated by the temperature in which the bacterium is living.  Outer membrane proteins (omps) are produced when the bacterium lives inside a human or rodent host (37C), but not when the bacterium lives in the flea host (23C).  A hypothetical <I>omp</I> operon is shown above.  This hypothetical operon works similarly to the <I>lac</I> operon (for example, ompI is the inhibitor that binds the operator to repress transcription of <I>ompA-C</I>).  In this system there is no inducer.  The rise in temperature (from 23C to 37C) causes a structural change in ompI so that it released from the operator.  Match the following mutants (super-repressor, nonfunctional repressor, operator mutant, mutant in a Shine-Dalgarno sequence) to their likely results at 23C and 37C.  (+ = protein present, - = protein absent)</div> <bR> <div> <TABLE BORDER="2" CELLPADDING="1" CELLSPACING="1" WIDTH="642" BORDERCOLORLIGHT="#C0C0C0" BORDERCOLORDARK="#808080" FRAME="BOX" RULES="ALL" ALIGN="BOTTOM" HSPACE="0" VSPACE="0" > <tR> <tD VALIGN=TOP HEIGHT= 19 WIDTH="96"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="96" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=TOP COLSPAN=3 WIDTH="176"><div> 23C</div> </tD> <tD VALIGN=TOP COLSPAN=3 WIDTH="188"><div> 37C</div> </tD> <tD VALIGN=TOP ROWSPAN=3 WIDTH="149"><div> Restore wildtype regulation with plasmid-encoded wildtype DNA?</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 19 WIDTH="96"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="19" ALIGN="bottom" WIDTH="96" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=TOP WIDTH="62"><div> OmpA</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> OmpB</div> </tD> <tD VALIGN=TOP><NOBR><div> OmpC</div> </NOBR></tD> <tD VALIGN=TOP WIDTH="60"><div> OmpA</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> OmpB</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> OmpC</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 38 WIDTH="96"><div> Wildtype</div> <bR> </tD> <tD VALIGN=TOP WIDTH="62"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="48"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 23 WIDTH="96"><div> Mutant 1</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="48"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="149"><div> No</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 23 WIDTH="96"><div> Mutant 2</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="48"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="149"><div> Yes</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 23 WIDTH="96"><div> Mutant 3</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="48"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="149"><div> No</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 23 WIDTH="96"><div> Mutant 4</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="48"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="62"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="60"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="149"><div> No</div> </tD> </tR> </TABLE></div> <bR> <div> 2. You have isolated 10 yeast strains that require tryptophan in the medium to live.  You wish to group them into complementation groups so you mate each cell to each of the other cells and determine whether they will now grow on - trp medium.  (- means no growth was observed, + means growth was observed).</div> <bR> <div> <TABLE BORDER="2" CELLPADDING="1" CELLSPACING="1" WIDTH="472" BORDERCOLORLIGHT="#C0C0C0" BORDERCOLORDARK="#808080" FRAME="BOX" RULES="ALL" ALIGN="BOTTOM" HSPACE="0" VSPACE="0" > <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><IMG BORDER="0" SRC="1x1.gif" HEIGHT="20" ALIGN="bottom" WIDTH="44" HSPACE="0" VSPACE="0"></tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M1</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M2</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M3</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M4</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M5</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M6</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M7</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M8</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>M9</B></div> </tD> <tD VALIGN=TOP WIDTH="44"><div> <B>M10</B></div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M1</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M2</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> -</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M3</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M4</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>-</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> <B>-</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M5</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M6</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M7</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M8</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M9</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> +</div> </tD> </tR> <tR> <tD VALIGN=TOP HEIGHT= 20 WIDTH="44"><div> <B>M10</B></div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> -</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="36"><div> +</div> </tD> <tD VALIGN=TOP WIDTH="44"><div> -</div> </tD> </tR> </TABLE></div> <bR> <div> a. How many different <I>trp</I> genes are represented in the table above? Note each group and all cells within the group.</div> <div> b.  Briefly describe a way you could clone the <B>normal</B> functional versions of these mutant <I>trp</I> genes.</div> </td> </tr></table></body>